Gene Expression Analysis of Host Immune Responses in rhesus macaques infected with SIVmac239 or deglycosylated live-attenuated SIV. Macaca mulatta

To find the differences in innate immune response between nonpathogenic and pathogenic SIV infection, we examined transcriptome in PBMCs from macaques infected with pathogenic or nonpathogenic SIV. Overall design: Juvenile macaques were intavenousy infected with SIVmac239 or ∆5G. Blood samples were collected at 1 week and 3 weeks pi. Total RNA from the PBMCs were used for the microarray analysis. cRNA was hybridized to a rhesus microarray (Agilent ver.2). The data were normalized per chip to the 75 th percentile expression level, then subjected to per gene to normalization to the median expression of uninfected animals.

为探究致病性与非致病性猴免疫缺陷病毒（SIV）感染所诱导的先天免疫应答差异，我们对感染致病性或非致病性SIV的猕猴外周血单个核细胞（PBMCs）的转录组开展了检测。整体实验设计：幼年猕猴经静脉注射分别感染SIVmac239毒株与∆5G毒株，于感染后1周和3周采集血液样本。提取外周血单个核细胞中的总RNA用于基因芯片分析，将合成的互补RNA（cRNA）与恒河猴基因芯片（安捷伦Agilent 版本2）进行杂交。数据首先以每块芯片的75百分位表达水平进行标准化处理，随后针对每个基因，以未感染动物的表达中位数完成二次标准化。