RNA splicing and aggregate gene expression differences between lung squamous cell carcinoma from patients of West African and European ancestry

Study of genes that are differentially spliced and differentially expressed between African Americans and whites with lung squamous cell cancer. Despite racial disparities in lung cancer, the molecular landscape of lung cancer in patients of African ancestry remains underexplored. Population-related differences in alternative RNA splicing have not been explored. We identified differentially spliced genes and differentially expressed genes between lung squamous cell carcinoma from patients of West African and European ancestry. RNA extracted from 20 African American and 20 white resected lung tissue was run on Clariom D exon array. Analysis to determine differences between population groups was performed using Transcriptome Analysis Console Software 4.0 (Affymetrix).

本研究针对肺鳞状细胞癌患者中非洲裔美国人与白人之间的差异剪接基因与差异表达基因展开探究。尽管肺癌存在种族差异，但非洲血统肺癌患者的肿瘤分子图谱仍未得到充分探索，群体相关的RNA可变剪接差异亦尚未被研究。我们在西非血统与欧洲血统患者的肺鳞状细胞癌样本中鉴定出差异剪接基因与差异表达基因。从20例非洲裔美国人和20例白人的切除肺组织中提取的RNA，通过Clariom D外显子芯片（Clariom D exon array）进行检测。采用Affymetrix公司的转录组分析控制台软件4.0（Transcriptome Analysis Console Software 4.0）完成了群体间差异分析。