Intracellular Staphylococcus aureus perturbs the host cell Ca2+-homeostasis to promote cell death

The pathogen Staphylococcus aureus can invade and survive within human cells. There, the intracellular bacterium induces host cell death, which may facilitate spread of infection and tissue destruction. We here conducted a shRNA screen and infected the library of knock-down cells with S. aureus. We identified calcium signaling pathways to play a role in S. aureus invasion and cytotoxicity. For latter, the intracellular bacteria induce a cytoplasmic and mitochondrial Ca2+-overload, which results in host cell death. HeLa shRNA Screen

病原菌金黄色葡萄球菌（Staphylococcus aureus）可侵入并存活于人类细胞内。在胞内环境中，该菌可诱导宿主细胞死亡，这或许会促进感染扩散与组织损伤。本研究开展了短发卡RNA（shRNA）筛选实验，使用金黄色葡萄球菌（S. aureus）感染敲低细胞库，最终鉴定出钙信号通路在金黄色葡萄球菌侵入宿主细胞及诱导细胞毒性过程中发挥关键作用。针对细胞毒性这一过程，胞内细菌可诱导细胞质与线粒体发生钙超载，进而引发宿主细胞死亡。HeLa细胞shRNA筛选