Canine peripheral blood TCRαβ T cell atlas: Identification of diverse subsets including CD8+ MAIT-like cells by combined single-cell transcriptome and V(D)J repertoire analysis

The diversity of canine T cells is poorly understood. We used 5’ scRNA-seq coupled with immune repertoire sequencing to generate a highly detailed atlas of peripheral blood TCRαβ+ T cells of healthy dogs. FACS-purification of TCRab T cells from PBMC of 4 healthy Beagle dogs based on TCRab expression followed by 5' scRNAseq with V(D)J analysis

犬类T细胞的多样性目前尚未得到充分阐明。本研究采用5'单细胞RNA测序（5’ scRNA-seq）结合免疫组库测序技术，构建了健康犬外周血TCRαβ+ T细胞的高精度详细图谱。研究人员基于TCRαβ表达特征，从4只健康比格犬的外周血单个核细胞（PBMC）中通过荧光激活细胞分选术（FACS）纯化出TCRαβ T细胞，随后开展了带有V(D)J重组分析的5'单细胞RNA测序。