Identification of TBBPA-degraders in anaerobic bioreactor at environmentally relevant concentrations by combined protein-based stable isotope probing and metagenomics

The aim of this study was to identify TBBPA-degrading organisms in a complex microbial community by a metagenome-based functional metaproteomic approach, using protein-based stable isotope probing (protein-SIP). Firstly, the degradation kinetics were evaluated in order to simulate the decrease of residual mass of the labelled compound based on experimental data. In sequence, a metagenome was generated, and biomass was collected in different time-points for protein-SIP in incubations with 13C-TBBPA. This approach allowed for the identification organisms assimilating labelled carbon from the cometabolic degradation of a micropollutant.

本研究旨在通过基于宏基因组（metagenome）的功能宏蛋白质组学方法，结合蛋白质稳定同位素探针（protein-SIP）技术，从复杂微生物群落中筛选可降解四溴双酚A（TBBPA）的微生物。首先，基于实验数据评估降解动力学，以模拟标记化合物的残留质量衰减过程。随后，获取宏基因组数据，并在添加13C标记四溴双酚A（13C-TBBPA）的培养体系中，于不同时间节点收集生物量用于protein-SIP分析。该策略可实现对通过微污染物共代谢降解途径同化标记碳的微生物的精准识别。