Genome-wide analysis of prostatic tissue gene expression from patients with benign prostatic hyperplasia. Homo sapiens

Analysis of gene expression in prostatic tissue from BPH patients with and without SRD5A2 gene methylation. The hypothesis is that BPH patients with DNA methylation of the SRD5A2 gene promoter have impaired conversion of testosterone to dihydrotestosterone, and therefore may use an alternative signaling pathway for prostatic tissue growth. Here, we compare gene expression profiles of SRD5A2-methylated vs. unmethylated prostatic tissue to nominate alternative biological pathways relevant in each molecular subtype of BPH. Overall design: 22 unique patient samples were analyzed (12 SRD5A2-methylated, 10 SRD5A2-unmethylated). All samples were transitional zone prostatic tissue obtained during transurethral resection of prostate (TURP) surgery.

针对伴与不伴SRD5A2基因甲基化的良性前列腺增生（Benign Prostatic Hyperplasia, BPH）患者的前列腺组织，开展基因表达分析。
本研究提出的假说为：SRD5A2基因启动子（promoter）区域发生DNA甲基化的BPH患者，其睾酮（testosterone）向二氢睾酮（dihydrotestosterone）的转化过程受损，因此可能通过替代信号通路驱动前列腺组织生长。
本研究通过对比SRD5A2甲基化与非甲基化前列腺组织的基因表达谱，旨在筛选与各BPH分子亚型相关的替代生物学通路。
实验设计概述：共分析22例独立患者样本（其中SRD5A2甲基化样本12例，非甲基化样本10例）。所有样本均取自经尿道前列腺切除术（Transurethral Resection of Prostate, TURP）术中获取的前列腺移行区组织。