Large scale cell-based transcriptome data treated with Jakyakgamcho-tang

Whole transcriptomic analysis in C2C12 cells was conducted, comparing cells treated with a vehicle control to those treated with three different concentrations of JGT-E and JGT-W. All experiments were performed in triplicate. Overall design: we constructed the pharmacological transcriptome data obtained through combinatorial conditions, such as extraction orders (individual herbs extracted or the herb-mixed JGT extracted), combination ratios (two herbs into 2:1, 1:1, and 1:2), two extracting methods (hot water or 70% ethanol), and three cell lines (muscle-related C2C12, neuropathic pain-related PC12, and drug metabolism-related HepG2).

本研究在C2C12细胞中开展全转录组分析（whole transcriptomic analysis），以经载体对照处理的细胞为对照组，与分别施加三种不同浓度JGT-E和JGT-W处理的细胞进行对比。所有实验均重复三次。

实验整体设计：本研究构建了通过多维度组合条件获取的药理转录组数据集，相关组合条件包括：提取次序（单味药材提取或复方JGT提取）、配伍比例（两味药材按2:1、1:1及1:2配比）、两种提取方法（热水提取或70%乙醇提取），以及三类细胞系：肌源性C2C12细胞、神经病理性疼痛相关PC12细胞、药物代谢相关HepG2细胞。