affy_med_2011_10- Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) line and in the lyr3-1 and lyr3-2 mutants

affy_med_2011_10 - affy_med_2011_10 - LYR3 encodes a lysin motif-receptor-like kinase (LysM-RLK) that consists of an extracellular ligand-binding domain composed of three LysM domains, a single transmembrane domain and a cytoplasmic serine/threonine kinase domain. A reverse genetic approach was undertaken to study the role of this gene in the pathosystem Medicago truncatula – Aphanomyces euteiches (Ae). Results suggest that lyr3-1 and lyr3-2 mutants are more susceptible to the root pathogen Aphanomyces euteiches than the A17 wild type (WT) line. In this study, we want to compare the early plant responses, 3 days post inoculation (dpi), between WT and the lyr3-1 and lyr3-2 mutants, in order to identify LYR3-dependent gene networks during infection.-Fourteen days old A17, lyr3-1 or lyr3-2 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = controls) with A. euteiches zoospores (105/ml) and harvested three days after inoculation. Three independent repeats were performed. 18 arrays - Medicago; normal vs mutant comparison,near isogenic lines (hif)

本数据集为affy_med_2011_10。
LYR3编码一种赖氨酸基序受体样激酶（LysM-RLK），该蛋白包含由3个LysM结构域组成的胞外配体结合结构域、单个跨膜结构域以及胞质丝氨酸/苏氨酸激酶结构域。本研究采用反向遗传学方法，探究该基因在蒺藜苜蓿（Medicago truncatula）-苜蓿根腐霉（Aphanomyces euteiches，简称Ae）这一寄主-病原互作系统中的功能。实验结果显示，与A17野生型（WT）株系相比，lyr3-1和lyr3-2突变体对该根部病原菌苜蓿根腐霉的敏感性显著高于野生型。
本研究旨在对比接种后3天（days post inoculation，dpi）时野生型与lyr3-1、lyr3-2突变体的早期植物响应，以鉴定侵染过程中依赖LYR3的基因调控网络。
实验步骤如下：将生长14天的A17、lyr3-1或lyr3-2植株在M培养基上进行体外培养。随后对全部根系接种（未接种组作为对照组）浓度为10^5/ml的苜蓿根腐霉游动孢子，并于接种后3天收获样本。本实验共设置3次独立生物学重复。
本数据集包含18个苜蓿基因芯片样本，用于野生型与突变体的比较分析，所用材料为近等基因系（HIF）。