Effect of metabolites supplementation on C2C12 gene expression

To investigate the effects of 1C-metabolite cocktail on C2C12 differentiated myofibers in vitro and determine the changes in gene expression. Particularly looking at potential insights of what the metabolite intervention induces in vitro We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different conditions including control myofibers untreated, myofibers treated with 1C-MIM, and myofibers treated with a scramble metabolite supplementation Overall design: Comparative gene expression profiling analysis of RNA-seq data for C2C12 differentiated myofibers and the conditions in which were supplemented

为探究一碳代谢物混合物（1C-metabolite cocktail）对体外（in vitro）培养的C2C12分化肌纤维的作用，并明确基因表达的变化情况，本研究重点关注该代谢物干预在体外环境下所诱导的潜在调控效应。随后，我们基于三种不同实验条件下获取的RNA测序（RNA-seq）数据开展基因表达谱分析，这三种条件分别为：未处理的对照肌纤维、经1C-MIM处理的肌纤维，以及经随机代谢物补充处理的肌纤维。实验设计概述：针对C2C12分化肌纤维及其经不同补充干预条件的RNA测序数据，开展对比性基因表达谱分析。