Chip-chip from Mouse MEFs cells for H3K36me3, Srsf1, Psip1(A300-847 antibody).. Mus musculus

The p52 isoform of Psip1/Ledgf links histone H3K36 methylation and the regulation of alternative splicing. Chromatin immunoprecipitation (ChIP) of Psip1 together with H3K36me3 and Srsf1 and by ChIP-on-chip analysis demonstrated that H3K36me3, Psip1 and SRSF1 enrichment correlates on the gene bodies Overall design: Array design includes 2 dye swap replicates for Srsf1 and Psip1-/- samples, and single arrays for PSIP and H3K36me3 samples

Psip1/Ledgf的p52亚型可介导组蛋白H3K36甲基化与可变剪接调控之间的关联。通过对Psip1、H3K36me3及Srsf1进行染色质免疫共沉淀（Chromatin immunoprecipitation, ChIP）并结合ChIP-on-chip分析，研究证实H3K36me3、Psip1与SRSF1的富集区域在基因体上呈现相关性。实验设计概述：本芯片阵列的设计包含针对Srsf1与Psip1基因敲除（Psip1-/-）样本的2组染料交换重复样本，以及针对PSIP和H3K36me3样本的单张芯片。