ATAC sequencing of zebrafish pancreatic beta-cells

Pancreatic beta-calls are responsible for regulating the blood glucose levels via secretion of hormone insulin. To elucidate the chromatin state in zebrafish beta-cells, we performed ATAC-Sequencing. Two replicates of beta-cells. For control, one replicate of hepatocytes was generated. ATAC-Seq was performed on 50k FACS sorted cells obtained from 3 mpf animals. For beta-cells, Tg(ins:Brainbow1.0) was used, while for hepatocytes, Tg(fabp10:dsRed, ela3l:GFP)gz12, also known as 2CLIP (2-color liver pancreas), was used.

胰腺β细胞通过分泌胰岛素调节机体血糖水平。为解析斑马鱼β细胞的染色质开放状态，本研究开展了ATAC测序（ATAC-Seq）实验。实验共设置两组斑马鱼β细胞生物学重复，并以肝细胞作为对照，设置一组生物学重复。所有实验细胞均取自3月龄（受精后月数，months post fertilization，简称mpf）的斑马鱼个体，通过荧光激活细胞分选（FACS，Fluorescence-Activated Cell Sorting）获得5×10^4个分选后细胞用于ATAC测序。其中，β细胞分选使用Tg(ins:Brainbow1.0)转基因品系，肝细胞分选则使用Tg(fabp10:dsRed, ela3l:GFP)gz12品系，该品系亦被称为2CLIP（双色肝胰标记系统，2-color liver pancreas）。