Single cell RNA-sequencing of Spike-ins and Control RNA using 10x Genomics Chromium system

In this study, we assess technical differences between commonly used single-cell RNA-Sequencing (scRNA-Seq) methods. In this dataset, we assess the RNA detection rates using high-throughput 10x Genomics Chromium system. We mix equal volume of Control Brain RNA (3µl; FirstChoice Total Brain RNA; #AM7962) and ERCC spikes (3µl 1:4 dilution; #4456653) to make a ‘2x Control RNA+ERCC’ master mix. The ‘2x Control RNA+ERCC’ master mix is diluted with equal volume nuclease-free water to make ‘1x Control RNA+ERCC’ master mix. 3µl of ‘1x Control RNA+ERCC’ master mix is added to Chromium single cell suspension and processed as per Chromium guidelines.

本研究旨在评估常用单细胞RNA测序（single-cell RNA-Sequencing, scRNA-Seq）方法之间的技术差异。本数据集通过高通量10x Genomics Chromium系统评估RNA检测率。我们将等体积的对照脑RNA（3μl；FirstChoice Total Brain RNA；货号#AM7962）与ERCC spike-in（3μl 1:4稀释液；货号#4456653）混合，制备成‘2x Control RNA+ERCC’母液。将‘2x Control RNA+ERCC’母液用等体积无核酸酶水稀释，制备成‘1x Control RNA+ERCC’母液。取3μl‘1x Control RNA+ERCC’母液加入Chromium单细胞悬液中，并按照Chromium操作指南进行处理。