Expression data from human macrophages. Homo sapiens

Human CD14 positive monocytes were purified from healthy volunteers’ blood and cultured in vitro for 4, 12, 24, 72 hours. While culturing, macrophages were activated alternatively with interleukin-4 (IL-4 100 ng/ml) or classically with interferon-gamma (IFNg 100 ng/ml)+tumor necrosis factor (TNF 50 ng/ml) or left without activation. Simultaneously, macrophages were also treated with vehicle (DMSO:ethanol) or 1mM synthetic PPARg agonist, Rosiglitazone. We used Affymetrix microarrays (U133Plus 2.0) to analyze activation and PPARg-induced gene expression changes. Overall design: Monocytes from 3 donors were used and treated as indicated in Summary.

本数据集的实验样本为从健康志愿者血液中纯化分离的人类CD14阳性单核细胞（Human CD14 positive monocytes），将其体外培养4、12、24、72小时。培养期间，分别采用白细胞介素-4（IL-4，100 ng/ml）对巨噬细胞进行替代性活化，或采用干扰素-γ（IFN-γ，100 ng/ml）+肿瘤坏死因子（TNF，50 ng/ml）进行经典活化，同时设置未活化对照组。此外，各组巨噬细胞还分别经溶剂对照（二甲基亚砜:乙醇，即DMSO:ethanol）或1mM合成型过氧化物酶体增殖物激活受体γ（PPARγ）激动剂罗格列酮（Rosiglitazone）处理。本研究采用Affymetrix基因芯片（U133Plus 2.0）分析巨噬细胞活化及PPARγ诱导的基因表达变化。实验整体设计：本实验使用3名健康供者的单核细胞，按照摘要所述方案完成处理。