Cancer cell genetics shaping of the tumor microenvironment reveals myeloid cell-centric exploitable vulnerabilities in hepatocellular carcinoma [RNA-Seq]. Cancer cell genetics shaping of the tumor microenvironment reveals myeloid cell-centric exploitable vulnerabilities in hepatocellular carcinoma [RNA-Seq]

Hepatocellular carcinoma (HCC) is a highly heterogenous disease associated with an equally dynamic tumor microenvironment (TME). We generated somatic HCC mouse models bearing clinically-relevant oncogenic driver combinations that faithfully recapitulated different human HCC subclasses. Using RNA-seq data, we explored changes in the TME and dissected cancer cell intrinsic shapers of the TME. Overall design: mRNA profiles of bulk tumors, CD45+ cells and cancer cell lines from genetically-distinct HCC mouse models. 3-4 biological replicates.

肝细胞癌（hepatocellular carcinoma，HCC）是一种高度异质性疾病，伴随具有高度动态特征的肿瘤微环境（tumor microenvironment，TME）。本研究构建了携带临床相关致癌驱动基因组合的体细胞HCC小鼠模型，该模型可精准复现不同人类HCC亚型的病理特征。本研究利用RNA-seq数据，探究了肿瘤微环境的动态变化，并解析了肿瘤细胞内在的肿瘤微环境调控因子。
整体实验设计：来自遗传背景各异的HCC小鼠模型的实体瘤组织、CD45+免疫细胞及癌细胞系的mRNA表达谱，每组设置3-4次生物学重复。