Transcriptome analysis in transgenerational epigenetic inheritance. Transcriptome analysis in transgenerational epigenetic inheritance

The study was performed towards understanding transgenerational epigenetic inheritance at transcriptome level. The founder males of F0 generation (actin-GAL4/Pin; UASRNAi-mts/+), and the resulting, male-line derived F1 (Pin/+; +/+), and F2 (+/+; +/+) generation individuals were used as test lineage, and the wild-type w1118 progeny as control lineage flies. The fly strains used for generating founder F0 line included Pin/CyO-dYFP; UASRNAi-mts/MKRS, actin-GAL4/CyO; +/+, and w1118. Precursor strains, as appropriate, were outcrossed with w1118 to minimize genetic background differences. Overall design: F0 males (actin-GAL4/Pin; UASRNAi-mts) were mated with w1118 females to obtain F1 progeny (Pin/+; +/+), and F1 males mated with w1118 females to obtain F2 progeny (+/+; +/+) in the test lineage. In parallel, w1118 flies were self-crossed, and the control lineage flies obtained. Three to five biological replicates were used.

本研究旨在解析转录组水平的跨代表观遗传（transgenerational epigenetic inheritance）现象。F0代奠基雄蝇（actin-GAL4/Pin; UASRNAi-mts/+）、雄性谱系衍生的F1代（Pin/+; +/+）及F2代（+/+; +/+）个体作为测试谱系果蝇，野生型w1118后代作为对照谱系果蝇。用于构建F0奠基株系的果蝇品系包括Pin/CyO-dYFP; UASRNAi-mts/MKRS、actin-GAL4/CyO; +/+以及w1118。必要时，将亲本前体株系与w1118进行异系杂交以最小化遗传背景差异。实验整体设计如下：F0代雄蝇（actin-GAL4/Pin; UASRNAi-mts）与w1118雌蝇交配，获得F1后代（Pin/+; +/+）；F1代雄蝇再与w1118雌蝇交配，获得测试谱系的F2后代（+/+; +/+）。与此同时，野生型w1118果蝇自交获得对照谱系果蝇。本实验设置3至5次生物学重复。