Single cell transcriptomic analysis of skeletal muscle cells. Single cell transcriptomic analysis of skeletal muscle cells

Single cell RNA-sequencing was used to analyze the cellular heterogeneity of mononucleated, non-myofiber cell populations and their transcriptional states in post-injury skeletal muscle Overall design: Droplet-based scRNA-sequencing of non-myofiber mononucleated cells purified by fluorescence-activated cell sorting (FACS) from muscle samples of aged and young control female mice at 4 days post injury was performed.

本研究借助单细胞RNA测序（single cell RNA-sequencing）技术，解析损伤后骨骼肌内单核非肌纤维细胞群的细胞异质性及其转录状态。
实验设计：本研究针对损伤后4天的年轻与老年雌性对照小鼠的肌肉样本，对经荧光激活细胞分选（fluorescence-activated cell sorting, FACS）纯化得到的非肌纤维单核细胞，开展基于液滴的单细胞RNA测序。