Germination and vigor of soybean genotypes seeds under saline stress

ABSTRACT Soybean stands out among the crops with the greatest application of transgenics, mainly for tolerance to herbicides, pests, and diseases; however, studies on salt stress in genetically modified plants are scarce. This study aimed to evaluate the tolerance of both traditional and genetically modified soybean genotypes to saline stress during the germination and seedling phases. Seeds of 13 soybean genotypes were selected (five traditional (BRS Carnaúba, BRS Pérola, BRS Tracajá, BRS Sambaíba, and FTR-4389) and eight transgenic (BRS Sambaíba RR, BRS-333-RR, BRS-9820- RR, PAS-13565-74-RR, PAS-11711-007-RR, BRS-918-IPRO, AS-3810-IPRO, and M-8210-IPRO)), subjected to four osmotic potentials (0.0, 0.1, -0.2, and -0.3 MPa). The seed quality was evaluated using the following variables: the germination percentage, germination speed index, shoot length, root length, dry mass accumulation, and salinity tolerance index. All variables were found to be affected by salt stress. However, the conventional genotypes, BRS Carnaúba, BRS Pérola, BRS Tracajá, and BRS Sambaíba, and the RR group PAS-13565-74-RR, and PAS-11711-007-RR were tolerant to salinity, whereas the genotypes FTR-4389 (conventional) and BRS Sambaíba RR (RR) were less tolerant to salt stress, and all genotypes in the IPRO group were moderately tolerant to salt stress.

摘要：大豆是转基因应用最为广泛的作物之一，其转基因品种主要具备耐除草剂、抗虫害及抗病特性；但目前针对转基因作物盐胁迫的相关研究仍较为匮乏。本研究旨在评估传统与转基因大豆基因型在萌发期及幼苗期的盐胁迫耐受性。本试验选取13个大豆基因型的种子，其中5个为传统品种（BRS Carnaúba、BRS Pérola、BRS Tracajá、BRS Sambaíba及FTR-4389），8个为转基因品种（BRS Sambaíba RR、BRS-333-RR、BRS-9820-RR、PAS-13565-74-RR、PAS-11711-007-RR、BRS-918-IPRO、AS-3810-IPRO及M-8210-IPRO），并将其置于4种渗透势（0.0、0.1、-0.2及-0.3 MPa）条件下培养。本研究通过以下指标评估相关性状：发芽率、发芽速率指数、苗长、根长、干物质积累量及盐胁迫耐受指数。试验结果显示，所有测定指标均受盐胁迫的显著影响。其中，传统基因型BRS Carnaúba、BRS Pérola、BRS Tracajá及BRS Sambaíba，以及RR类群的PAS-13565-74-RR、PAS-11711-007-RR表现出较强的盐胁迫耐受性；传统基因型FTR-4389与转基因基因型BRS Sambaíba RR的盐胁迫耐受性较弱；IPRO类群的所有基因型则均表现出中等程度的盐胁迫耐受性。