SL-C-AMm-20210330-0031

Inflammatory bowel disease (IBD) is a chronic inflammatory disease with an increasing incidence year by year, and its pathogenesis may be related to factors such as dysbiosis of intestinal flora. Miao sour soup (MSS), a fermented food for daily consumption in Guizhou, China, is rich in microorganisms with many excellent activities, including anti-inflammatory and antioxidant activities. However, the therapeutic effects of MSS on IBD remain to be explored. The aim of this study was to investigate the protective effect of MSS against colitis in mice In this study, we investigated the community structure of MSS and the protective role of MSS in against dextran sulfate sodium salt (DSS)-induced colitis in mice. In this study, we examined the microbial community structure of MSS by metagenomic sequencing, and also explored the protective effect of MSS on DSS induced colitis in mice. We investigated the effects of MSS on intestinal inflammatory response and intestinal barrier function in mice. Finally, the changes of intestinal flora were analyzed based on the results of 16S rRNA gene sequencing.The results showed that MSS contained a variety of probiotics.

炎症性肠病（Inflammatory Bowel Disease，IBD）是一类发病率逐年上升的慢性炎症性疾病，其发病机制可能与肠道菌群失调等因素密切相关。苗家酸汤（Miao Sour Soup，MSS）作为中国贵州地区日常食用的发酵食品，富含多种具有优异生物活性的微生物，涵盖抗炎及抗氧化活性。然而，苗家酸汤针对炎症性肠病的治疗效果仍有待深入探究。本研究旨在考察苗家酸汤对小鼠结肠炎的保护作用：首先通过宏基因组测序解析了苗家酸汤的微生物群落结构，并探究了其对硫酸葡聚糖钠盐（Dextran Sulfate Sodium Salt，DSS）诱导的小鼠结肠炎的保护作用；随后分析了苗家酸汤对小鼠肠道炎症反应与肠道屏障功能的影响；最后基于16S核糖体RNA（16S rRNA）基因测序结果，解析了小鼠肠道菌群的变化情况。研究结果显示，苗家酸汤中含有多种益生菌。