CD4 and INFg mRNA/Protein expression in stimulated PBMC

The purpose was to demonstrate the effect of a 5 hour PMA/Ionomycin stimulation on expression of IFNg mRNA, CD4 mRNA, CD4 protein, and IFNg protein in lymphocytes.
Conclusion:
After a 5 hour PMA/Ionomycin treatment, expression of CD4 protein and mRNA is decreased, while both IFNg protein and mRNA is increased.
Notes:
Figure 2A-F and supplemental: Simultaneous flow cytometric analysis of IFN-g and CD4 mRNA and protein expression kinetics in human peripheral blood mononuclear cells during activation Cytometer setup using CST in FACSDiva software.

本实验旨在探究5小时佛波醇12-肉豆蔻酸酯13-乙酸酯（Phorbol 12-myristate 13-acetate, PMA）/离子霉素（Ionomycin）刺激对淋巴细胞中干扰素γ（Interferon-γ, IFNg）信使核糖核酸（messenger RNA, mRNA）、CD4 mRNA、CD4蛋白以及IFN-γ蛋白表达水平的影响。
结论：
经5小时PMA/离子霉素处理后，淋巴细胞中CD4蛋白及其mRNA的表达水平均下调，而IFN-γ蛋白与mRNA的表达水平均上调。
备注：
图2A-F及补充材料：人外周血单个核细胞激活过程中IFN-γ与CD4的mRNA及蛋白表达动力学的同步流式细胞术分析；采用FACSDiva软件结合CST完成流式细胞仪设置校准。