Metabolomic analysis of pheasantshell mussel (Ortmanniana pectorosa; Order Unionida) from a mass mortality event in the Clinch River, Virginia and Tennessee, USA

This dataset uses the ITIS recommended Ortmanniana pectorosa, Conrad, 1834 in place of Actinonaias pectorosa, which was used in the Richard et al. 2020 manuscript. Biologists monitoring freshwater mussel (Order Unionida) populations rely on behavioral, often subjective, symptoms to identify “sick” or stressed animals, such as gaping valves and slow response to probing and lack clinical indicators to support a diagnosis. As part of a multi-year study to investigate causes of reoccurring mortality of pheasantshell (Ortmanniana pectorosa, Conrad 1834) in the Clinch River, Virginia and Tennessee, USA, we analyzed the hemolymph metabolome of a subset of mussels from the 2018 sampling period. Mussels at the mortality sites were diagnosed as affected (case) or unaffected (control) based on behavioral and physical signs. Hemolymph was collected in the field by nonlethal methods from the anterior adductor muscle for analysis. We used ultra-high-performance liquid chromatography, quadrupole time-of-flight mass spectroscopy (UHPLC-QTOF-MS) to detect targeted and untargeted metabolites in hemolymph and compared metabolomic profiles by field diagnosis. This dataset contains information about the viral load measured previously by Richard et al. 2020. We also include the intensity of mass spectrum signals from metabololites found in the hemolymph of pheasantshell mussels (Ortmanniana pectorosa, Conrad, 1834). These values are unitless values as the intensity is measured as the area under the curve for each metabolite.

本数据集采用综合分类学信息系统（ITIS）推荐的*Ortmanniana pectorosa, Conrad, 1834*，替代Richard等人2020年手稿中使用的*Actinonaias pectorosa*。监测淡水蚌类（蚌目Unionida）种群的生物学家，通常依靠带有主观性的行为学症状来识别“患病”或应激个体，例如壳瓣张开、触碰后反应迟缓，但目前尚无支持该诊断的临床指标。作为针对美国弗吉尼亚州与田纳西州克林奇河内雉蚌（*Ortmanniana pectorosa, Conrad 1834*）反复死亡原因开展的多年研究的一部分，我们对2018年采样时段中部分蚌类的血淋巴代谢组进行了分析。研究人员依据行为与体表体征，将死亡监测点的蚌类划分为患病组（病例）与健康对照组，并通过非致死方法在野外从蚌类的前闭壳肌采集血淋巴用于后续分析。我们采用超高效液相色谱-四极杆飞行时间质谱（UHPLC-QTOF-MS）检测血淋巴中的靶向与非靶向代谢物，并根据野外诊断结果对比代谢组学特征。本数据集包含Richard等人2020年已测定的病毒载量相关信息，此外还收录了从雉蚌（*Ortmanniana pectorosa, Conrad, 1834*）血淋巴中检测到的代谢物的质谱信号强度。由于该强度以每种代谢物的峰下面积计算，因此这些数值均无单位。