Open chromatin in somatic cells transplanted into Xenopus oocytes. Open chromatin in somatic cells transplanted into Xenopus oocytes

Active gene transcription is associated with an open chromatin configuration at gene regulatory regions. However, the relationship between chromatin architecture of somatic cells and transcriptional activation during nuclear reprogramming in oocytes is unclear. We therefore examined changes in open chromatin states before and after transplantation of mouse somatic nuclei into Xenopus laevis oocytes by using the Assay for Transposase-Accessible Chromatin Sequencing (ATAC-seq). Overall design: ATAC-seq was performed using the following cell samples; (1) C2C12 cells, (2) mouse embryonic fibroblasts (MEFs) before and after nuclear transplantation into Xenopus laevis oocytes.

基因的活跃转录与基因调控区域的开放染色质构象密切相关。然而，卵母细胞核重编程过程中体细胞染色质结构与转录激活的关联仍不明确。为此，本研究借助转座酶可及性测序（Assay for Transposase-Accessible Chromatin Sequencing，ATAC-seq）技术，分析了小鼠体细胞核移植入非洲爪蟾（Xenopus laevis）卵母细胞前后的开放染色质状态变化。
实验设计概述：采用以下细胞样本开展转座酶可及性测序实验：(1) C2C12细胞；(2) 体细胞核移植入非洲爪蟾卵母细胞前后的小鼠胚胎成纤维细胞（mouse embryonic fibroblasts，MEFs）。