Gene expression profile at single cell level of CD11b+/CD45+ myeloid cells from the brain (scRNA-seq_female). Gene expression profile at single cell level of CD11b+/CD45+ myeloid cells from the brain (scRNA-seq_female)

We used single cell RNA sequencing (scRNA-seq) to analyze the subsets of myeloid cells affected by deletion of miR-155 in microglia. Overall design: CD11b+/CD45+ myeloid cells were isolated by Fluorescence-activated cell sorting (FACS) and analyzed using scRNAseq.

本研究采用单细胞RNA测序（single cell RNA sequencing，scRNA-seq）技术，分析小胶质细胞中miR-155敲除所影响的髓系细胞亚群。实验整体设计：通过荧光激活细胞分选术（Fluorescence-activated cell sorting，FACS）分离CD11b⁺/CD45⁺髓系细胞，并利用scRNA-seq完成分析。