Single cell analysis of MYD88 L265P and MYD88 WT Waldenström macroglobulinemia (WM) patients. Single cell analysis of MYD88 L265P and MYD88 WT Waldenström macroglobulinemia (WM) patients

To elucidate the differences occurring in the B cell composition between WM patients, we performed single cell RNA sequencing on CD19 + sorted cells from patients with MYD88 L265P versus MYD88 WT phenotype and compared them with two healthy controls. Overall design: Single cell RNA sequencing of CD19+ bone marrow sorted cells of WM patients and two healthy donors was performed on the 10x Genomics Chromium Illumina NovaSeq6000 platform according to standard procedures.

为阐明华氏巨球蛋白血症（Waldenström's Macroglobulinemia, WM）患者之间B细胞组成的差异，我们对携带MYD88 L265P突变与MYD88野生型（MYD88 WT）表型的WM患者的CD19阳性骨髓分选细胞进行了单细胞RNA测序，并与两名健康对照进行比较。整体实验设计：本研究按照标准流程，在10x Genomics Chromium与Illumina NovaSeq6000平台上，对WM患者及两名健康供者的CD19阳性骨髓分选细胞开展了单细胞RNA测序。