RNA-sequencing analysis of NB4 cells overexpressing miR-125b

To better understand the mechanisms of blockage of myeloid differentiation and apoptosis and induction of proliferation by miR-125b, we proceeded to identify miR-125b target genes involved in these pathways. We analyzed the total cellular gene expression pattern by RNA-sequencing of the parental NB4 myeloid cell line and that transiently transfected with miR-125b. We generated four cDNA libraries corresponding to duplicates of miR-125b and control cells. Compare the gene expression levels in miR control transfected cells with that in miR-125b transfected NB4 cells.

为更精准地阐明miR-125b介导髓系分化阻滞、凋亡抑制及增殖诱导的分子机制，我们拟筛选参与上述调控通路的miR-125b靶基因。本研究通过RNA测序（RNA-sequencing）分析亲本NB4髓系细胞系，以及经瞬时转染miR-125b的该细胞系的全细胞基因表达谱。我们共构建了4个cDNA文库，分别对应miR-125b转染组与对照组的生物学重复样本。本次研究将比较miRNA对照转染细胞与miR-125b转染NB4细胞的基因表达水平差异。