Comprehensive transcriptome analysis of heart tissues in the sugen/hypoxia rats with/without treatment of chrysin

Pulmonary arterial hypertension (PAH) is a progressive disease characterized by pulmonary vascular remodeling leading to increased pulmonary vascular resistance and right ventricular (RV) hypertrophy, resulting in RV failure. RV dysfunction is a complex process that leads to cardiomyocyte hypertrophy, fibrosis, inflammation, angiogenesis, and metabolic changes. In patient with PAH, the adaptation of the RV to high pulmonary arterial pressures is the most important determinant of prognosis. However, the underlying molecular mechanism of adverse RV remodeling and dysfunction is poorly understood, and there are no currently approved therapies that improve RV function. Chrysin (5,7-dihydroxyflavone) is a phytochemical, which is a flavonoid widely present in plant sources. In various experimental models chrysin has shown to exert cardio-protective effects through its antioxidant and anti-inflammatory effects. In this study, we performed a comprehensive analysis of gene expression changes in heart tissues of rats under hypoxic conditions with chrysin treatment using RNA sequencing (RNA-seq).肺动脉高压（Pulmonary arterial hypertension, PAH）是一种进行性疾病，以肺血管重构为特征，可导致肺血管阻力升高及右心室（right ventricular, RV）肥厚，最终引发右心衰竭。右心室功能异常是一个复杂的病理过程，可引发心肌细胞肥厚、纤维化、炎症反应、血管生成及代谢改变。在肺动脉高压患者中，右心室对高肺动脉压的适应能力是判断预后的最重要决定因素。然而，目前对于右心室不良重构与功能异常的潜在分子机制仍知之甚少，且尚无获批用于改善右心室功能的治疗手段。白杨素（Chrysin，5,7-二羟基黄酮）是一类广泛存在于植物中的黄酮类植物化学物，在多种实验模型中，其已被证实可通过抗氧化及抗炎作用发挥心脏保护功效。本研究采用转录组测序（RNA sequencing, RNA-seq）技术，全面分析了低氧条件下经白杨素处理的大鼠心脏组织的基因表达变化。