High tolerance and degradation of fungicides by fungal strains isolated from contaminated soils

The aim of this work was to isolate fungal strains from phytotoxic agricultural soils, screen them, categorize the most tolerant fungi to three fungicides, and identify them by a molecular approach. In this study, 28 fungal strains were isolated from phytotoxic agricultural soil with intensive use of pesticides. The capacity of fungi to resist and degrade different concentrations of carbendazim, captan, and zineb was determined by an exploratory multivariate analysis. <i>Actinomucor elegans</i> LBM 239 was identified as the most tolerant fungus to these fungicides, degrading a 86.62% of carbendazim after 7 days of treatment. In conclusion, <i>A. elegans</i> LBM 239 demonstrated the highest tolerance and capacity to biodegrade carbendazim, becoming a potential candidate for bioremediation of contaminated soils with carbendazim, captan, or zineb.

本研究旨在从具植物毒性的农业土壤中分离真菌菌株，经筛选后分类得到对三种杀菌剂耐受性最优的菌株，并通过分子生物学方法完成菌株鉴定。本研究从经高强度农药施用的具植物毒性农业土壤中，分离得到28株真菌菌株。本研究通过探索性多元分析，测定了真菌对不同浓度多菌灵（carbendazim）、克菌丹（captan）及代森锌（zineb）的耐受与降解能力。经鉴定，雅致放射毛霉（Actinomucor elegans）LBM 239为对上述三类杀菌剂耐受性最强的菌株，在培养7天后可降解86.62%的多菌灵。综上，雅致放射毛霉LBM 239展现出最高的耐受性及多菌灵生物降解能力，有望成为多菌灵、克菌丹、代森锌污染土壤生物修复的潜在候选菌株。