A low-cost and customizable alternative for commercial implantable cannula for intracerebral administration in mice

Repository of design files for the HardwareX article titled "A low-cost and customizable alternative for commercial implantable cannula for intracerebral administration in mice".

Contributing authors: Rogneda B Kazanskaya, Alexander V Lopachev, Tatiana N Fedorova, Raul R Gainetdinov, Anna B Volnova.

Abstract: Stereotaxic intracerebral cannula implantation for neuroactive agent administration is a wide-spread method for chronic experiments requiring bypassing the blood-brain barrier in rodents. However, commercially available cannula are bulky and may interfere with animal movement or lead to their dislodging during grooming. As the number of cannula needed in one experiment, and the accompanying costs can be high, it is in the interest of researchers to produce them on their own. Custom cannula manufacturing also offers the flexibility of different cannula lengths, which is required for agent delivery to various brain structures. In this article we present a protocol for making guide cannula along with the accompanying systems required for injection, which are small, cost-effective, light, easy to make, reusable, and can be made from easily procured materials.

面向《HardwareX》期刊论文《面向小鼠脑内给药的商用植入式套管（implantable cannula）的低成本定制化替代方案》的设计文件仓库。

参与作者：罗格涅达·B·卡赞斯卡娅（Rogneda B Kazanskaya）、亚历山大·V·洛帕切夫（Alexander V Lopachev）、塔季扬娜·N·费多罗娃（Tatiana N Fedorova）、劳尔·R·盖涅季诺夫（Raul R Gainetdinov）、安娜·B·沃尔诺娃（Anna B Volnova）。

摘要：用于递送神经活性物质的立体定向脑内套管植入术，是啮齿类动物中绕过血脑屏障开展慢性实验的常用方法。然而，市售套管体积庞大，可能会干扰动物活动，或在动物理毛时导致套管脱落。由于单次实验所需套管数量及配套成本往往较高，研究者自行制备套管具有现实意义。定制化套管生产还可灵活调整套管长度，以满足将物质递送至不同脑区的需求。本文提出了一种制作引导套管及其配套注射系统的方案，该方案制备的装置体积小巧、成本低廉、重量轻盈、易于制作且可重复使用，同时可通过易于获取的材料加工而成。