Modeling pre-implantation development using totipotent blastomere-like stem cells [ATAC-seq]

we have established TBLC-based differentiation systems to recapitulate the entire pre-implantation development Assay for transposase accessible chromatin with high-throughput sequencing in mESC sand mTBLCs Comparative chromatin accessibility analysis of more open and closed signals in ATAC-seq for mPSCs and mTBLCs

我们已建立基于TBLC的分化体系，用于重现完整的植入前发育进程。针对小鼠胚胎干细胞（mouse embryonic stem cells, mESC）与mTBLCs，我们实施了转座酶可及性高通量测序（Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing，ATAC-seq）。本研究针对小鼠多能干细胞（mouse pluripotent stem cells, mPSCs）与mTBLCs的ATAC-seq数据，对其中的显著开放与闭合染色质信号开展了染色质可及性比较分析。