Experiment 2 % coverage.xlsx

To determine how DEX treatment in WT and MKO mice leads to muscle atrophy, we measured muscle protein fractional synthesis rates using shot-gun LC-MS/MS analysis after <i>in vivo</i> ²H₂O labeling . Mice were treated with PBS or DEX for 10 days. During the final 7 days, mice were also labeled with ²H₂O. The sample size for wild type controls treated with PBS (WT-PBS, n=6), wild type treated with DEX (WT-DEX, n=6), MKO treated with PBS (MKO-PBS, n=5) and MKO treated with DEX (MKO-DEX, n=5). The rational for biological replicates of n=5 -6 were used to ensure accurate comparison among each group. Biological replicates were chosen to asses biological variability rather than sole reliance on technical replicates. Controls included WT-PBS treated mice (n=6). Two separate dynamic proteomic experiments were completed and combined for final data analysis. This consisted of experiment 1 that included WT-PBS (n=4) and WT-DEX (n=4). Experiment 2 included the final biological replicates of WT-PBS (n=2), WT-DEX (n=2), MKO-PBS (n=5) and MKO-DEX (n=5). The data sets were combined and annotated as described by the following.<br>Entry name, accession number, group number, sub group number, number of spectra, number of unique peptides, unique score, percent coverage, coverage map, total intensity, protein molecular weight, database, parent charge, score, sequence, chromatographic peak width, parent mass to charge ratio, delta parent mass and delta parent mass in ppm are shown.<br>

为明确野生型（WT）与MKO小鼠经地塞米松（DEX）处理后诱发肌肉萎缩的机制，我们采用体内（in vivo）氘代水（²H₂O）标记结合鸟枪液相色谱-串联质谱（shot-gun LC-MS/MS）分析，测定肌肉蛋白质的分数合成速率。
小鼠经磷酸盐缓冲液（PBS）或DEX处理10天。在给药的最后7天，小鼠同时接受²H₂O标记。
各组样本量设置如下：PBS处理的野生型对照组（WT-PBS，n=6）、DEX处理的野生型组（WT-DEX，n=6）、PBS处理的MKO组（MKO-PBS，n=5）以及DEX处理的MKO组（MKO-DEX，n=5）。
本研究采用n=5~6的生物学重复，旨在确保各组间的准确比较。选择生物学重复而非仅依赖技术重复，是为了评估生物学变异。对照组为WT-PBS处理小鼠（n=6）。
本研究共完成2次独立的动态蛋白质组学实验，最终合并数据进行分析。实验1包含WT-PBS（n=4）与WT-DEX（n=4）组；实验2包含剩余的生物学重复：WT-PBS（n=2）、WT-DEX（n=2）、MKO-PBS（n=5）及MKO-DEX（n=5）。
合并后的数据集将按以下条目进行注释：条目名称、登录号、组编号、亚组编号、光谱计数、独特肽段数、独特得分、覆盖率百分比、覆盖率图谱、总强度、蛋白质分子量、数据库来源、母离子电荷、得分、氨基酸序列、色谱峰宽、母质荷比、母离子质量偏差及以ppm为单位的母离子质量偏差。