Effects of 4-HDHA on gene expression in colonic tissue of colitis mice

To investigate the roles of 4-HDHA in the regulation of cell function and gene expression of colonic tissue, we treated colitis mice with 4-HDHA intravenously. Then, we performed gene expression profiling analysis using data obtained from RNA-seq of colonic tissue of colitis mice treated with or without 4-HDHA. Overall design: Comparative gene expression profiling analysis of RNA-seq data for colons from adult C57BL/6 mice that were treated with 3% DSS continuously for 7 days. The mice were divided into two groups:one group was treated with PBS( 200ul/20g/day ) intravenously and the other group was treated with 5um 4-HDHA (diluted in PBS,200ul/20g/day) intravenously .The injection was along with 3% DSS for 7 days.

为探究4-HDHA在结肠组织细胞功能调控与基因表达中的作用，我们通过静脉注射方式对结肠炎小鼠给予4-HDHA处理。随后，基于经4-HDHA处理与未处理的结肠炎小鼠结肠组织的RNA测序（RNA-seq）数据，开展基因表达谱分析。
整体实验设计：本研究以连续给予3%葡聚糖硫酸钠（DSS）处理7天的成年C57BL/6小鼠的结肠组织RNA测序（RNA-seq）数据为研究对象，开展对比基因表达谱分析。将小鼠分为两组：一组经静脉注射磷酸盐缓冲液（PBS，200μl/20g/天），另一组经静脉注射5μM 4-HDHA（溶于PBS中，200μl/20g/天），注射操作与3% DSS处理同步进行，持续7天。