Human THP1 macrophages after interacting with C. albicans, using ATP-binding protein enrichment strategy

Macrophages are involved in the primary human host response to pathogens such as Candida albicans. After pathogen recognition by specific receptors, a cascade of signaling pathways is activated, leading to the production of cytokines, chemokines and antimicrobial peptides. ATP binding proteins are crucial for the regulation of signaling pathways. Taking into account the fact that proteomics can provide important information regarding these molecular mechanisms, a quantitative proteomic approach was carried out for the study of human macrophage ATP-binding proteins after interaction with C. albicans. A total of 547 non-redundant proteins were quantified in at least two biological replicates and with a standard deviation lower than 0.3. Among them, 59 proteins were considered differentially abundant during macrophage interaction with C. albicans. Moreover, with the proteins enriched in ATP-binding proteins a phosphopeptide enrichment was performed, and a total of 70 phosphosites was quantified, using the same filtering criteria. We repeated the search of the .raw files in the new nexprot database (NextProt v2.15.0, 20230 protein entries) that we also uploaded in this project. In summary, in this project we uploaded: a. Quantitative proteomic results- .raw files; .msf files; mgf and excel table with quantitative results b. Quantitative phosphor results - .raw files; .msf files; mgf and excel table with quantitative results c. Identification results with recent version of Nexprot-mzIdentML.

巨噬细胞参与人类宿主对白色念珠菌（Candida albicans）等病原体的初次免疫应答。当通过特异性受体识别病原体后，一系列信号通路被激活，进而介导细胞因子、趋化因子及抗菌肽的产生。ATP结合蛋白（ATP binding proteins）对信号通路的调控至关重要。考虑到蛋白质组学（proteomics）能够为阐明这类分子机制提供重要信息，本研究采用定量蛋白质组学方法，对人类巨噬细胞与白色念珠菌相互作用后的ATP结合蛋白进行了研究。最终在至少两次生物学重复实验中，共定量到547个非冗余蛋白，且各蛋白的标准差均低于0.3。其中，59个蛋白在巨噬细胞与白色念珠菌相互作用过程中呈现丰度差异。此外，针对富集得到的ATP结合蛋白组分，本研究还开展了磷酸肽富集分析，并采用相同的筛选标准，共定量到70个磷酸化位点（phosphosites）。我们还在本次项目中上传的新版NextProt数据库（NextProt v2.15.0，收录20230个蛋白质条目）内，对.raw文件重新进行了检索。综上，本项目上传的数据包括：
a. 定量蛋白质组学结果：.raw文件、.msf文件、.mgf文件及包含定量结果的Excel表格；
b. 定量磷酸化蛋白质组学结果：.raw文件、.msf文件、.mgf文件及包含定量结果的Excel表格；
c. 基于最新版NextProt数据库的鉴定结果：mzIdentML格式文件。