Methanogen mcrA NGS amplicon data - Higher methane emissions appear an inevitable consequence of natural warming

Targeted amplicon NGS data for functional microbial target genes to characterise methanogens (mcrA) and methanotrophs (pmoA) that drive methanogenesis.Sediment samples collected from high latitude streams across 5 sites during 2016 and 2017. Locations were Iceland, Alaska, Greenland (2016), Kamchatka, Svalbard (2017).These sequences will need to be split by as they contain multiple amplicons per run. The primer set to extract the data for methanogens needs to be completed bioinformatically.Forward primer GGYGGTGTMGGDTTCACMCARTAAngel et al 2011 Methanogenic archaea are globally ubiquitous in aerated soils and become active under wet anoxic conditions The Isme Journalmlas-mod is only 1 bp different from original mlas forward primer by Steinberg and Regan 2008 see below for reference.Reverse primer CGTTCATBGCGTAGTTVGGRTAGTSteinberg and Regan 2008 Phylogenetic comparison of the methanogenic communities from an acidic, oligotrophic fen and an anaerobic digester treating municipal wastewater sludge Applied and Environmental Microbiology

本数据集为靶向扩增子二代测序（NGS）数据，用于表征驱动产甲烷作用的产甲烷古菌（methanogens）的mcrA基因与甲烷氧化菌（methanotrophs）的pmoA基因这两类功能微生物靶标基因。研究样本为2016至2017年间采集自5处高纬度溪流的沉积物样品，采样点覆盖冰岛、阿拉斯加、格陵兰（2016年）以及堪察加半岛、斯瓦尔巴群岛（2017年）。由于单次测序运行中包含多种扩增子，需对这批序列进行拆分处理。用于提取产甲烷菌相关数据的引物组需通过生物信息学方法完成预处理。正向引物序列为GGYGGTGTMGGDTTCACMCARTA，源自Angel团队2011年的研究《产甲烷古菌在通气土壤中全球广泛分布并在湿润缺氧条件下被激活》，发表于《国际微生物生态学会会刊》（The Isme Journal）。mlas-mod引物与Steinberg和Regan于2008年报道的原始mlas正向引物仅相差1个碱基对，具体参考文献信息详见下文。反向引物序列为CGTTCATBGCGTAGTTVGGRTAG，源自Steinberg与Regan 2008年的研究《酸性贫营养沼泽与处理市政污水污泥的厌氧消化池中产甲烷菌群的系统发育比较》，发表于《应用与环境微生物学》（Applied and Environmental Microbiology）。