Supplemental Tables - Accelerated osteocytic citrate production in chronic kidney disease is associated with protection of the kidney

Supplemental Tables for manuscript: "Accelerated osteocytic citrate production in chronic kidney disease is associated with protection of the kidney".

Datasets:
Table S1: Differentially expressed genes in tibia cortices of control and AdKI mice (n=3 per group), analyzed via RNASeq and the Deseq2 algorithm.
Table S2: Extracellular 13C-labeling of metabolites within spent media of osteocyte-enriched femora (Ocy-Fem) and osteocyte-enriched calvariae (Ocy-Cal) following 24h of ex vivo culture in [1,2-13C]-glucose (n=5 per group).
Table S3: Intracellular 13C-labeling of metabolites extracted from osteocyte-enriched femora (Ocy-Fem) and osteocyte-enriched calvariae (Ocy-Cal) following 24h of ex vivo culture in [1,2-13C]-glucose (n=5 per group). Note: At the start (time 0h), it is assumed that M0 fragments predominate (100%) and no 13C-enrichment has taken place yet. Data at 9h and 24h are extracted from ex vivo cultured osteocyte-enriched bone organs.
Table S4: Extracellular 13C-labeling of metabolites within spent media of osteocyte-enriched femora (Ocy-Fem) following 24h of ex vivo culture in [1,2-13C]-glucose or [U-13C]-glutamine (n=5 for control and AdKI WT mice; n=4 for control and AdKI Slc13a5R337*/R337* mice).

(Accepted manuscript for publication in a peer-reviewed journal may differ)

学术论文《慢性肾脏病中骨细胞枸橼酸盐生成加速与肾脏保护相关》的补充表格

数据集：
表S1：对照组与AdKI小鼠胫骨皮质骨的差异表达基因（每组n=3），采用RNA测序（RNA-Seq）与DESeq2算法进行分析。
表S2：富集骨细胞的股骨（Ocy-Fem）与富集骨细胞的颅盖骨（Ocy-Cal）在[1,2-13C]-葡萄糖中完成24小时体外培养后，其培养上清液内代谢物的细胞外13C标记情况（每组n=5）。
表S3：富集骨细胞的股骨（Ocy-Fem）与富集骨细胞的颅盖骨（Ocy-Cal）在[1,2-13C]-葡萄糖中完成24小时体外培养后，从组织中提取的代谢物的细胞内13C标记情况（每组n=5）。注：培养初始阶段（0小时），假定以M0片段为主（占比100%），尚未发生13C富集。9小时与24小时的数据集取自体外培养的富集骨细胞骨组织。
表S4：富集骨细胞的股骨（Ocy-Fem）分别在[1,2-13C]-葡萄糖或[U-13C]-谷氨酰胺中完成24小时体外培养后，其培养上清液内代谢物的细胞外13C标记情况（对照组与AdKI野生型小鼠每组n=5；对照组与AdKI Slc13a5R337*/R337*突变小鼠每组n=4）。

（同行评审期刊录用的最终定稿或与本版本存在差异）