Effect of ppGpp on Salmonella Typhimurium gene expression. Effect of ppGpp on Salmonella Typhimurium gene expression

The expression profile of an S. Typhimurium mutant strain unable to synthesise ppGpp (relAspoT deletions) was compared to the wild-type strain. The effect of ppGpp on virulence gene expression was studied under 4 different growth conditions that induce virulence gene expression. Keywords: genetic modification Overall design: Study comprised 4 separate experiments (with 2 strains and 4 replicates for each strain per growth condition). The experiments were indirect comparisons using Salmonella genomic DNA as the comparator which also acted as the control for spot quality.

将无法合成ppGpp的鼠伤寒沙门氏菌（S. Typhimurium）relA与spoT双缺失突变菌株的基因表达谱与野生型菌株进行对照比较。本研究探究了ppGpp在4种可诱导毒力基因表达的不同生长条件下，对毒力基因表达的调控作用。
关键词：遗传修饰
总体设计：本研究包含4组独立实验，每种生长条件下设置2种菌株，且每种菌株各设4次生物学重复。实验采用间接对照设计，以沙门氏菌基因组DNA作为参比对照样品，同时用于斑点质量的质控验证。