RNA seq of prostate gland from WT, PTEN pc -/- and PTEN pc-/-;P53 -/- mice. RNA seq of prostate gland from WT, PTEN pc -/- and PTEN pc-/-;P53 -/- mice

Acute Pten loss initiates prostate tumorigenesis characterized by cellular senescence response. Senescent cells secrete a variety of pro inflammatory factors in the tumor microenvironment, which can support the survival, outgrowth and migration of tumor cells. Here we examine cytokines and cytokines-related factors gene expression in Ptenpc-/- senescent and in Ptenpc-/-; Trp53pc-/- non senescent tumors. RNAseq analysis confirmed the presence of cytokines, which were specifically up- and down-regulated in Ptenpc-/- senescent tumors (“core-SASP”) we also found a number of upregulated secreted factors that were “senescence-unrelated” both present in both Ptenpc-/- and Ptenpc-/-; Trp53pc-/- tumors.

急性PTEN缺失可启动以细胞衰老应答为特征的前列腺肿瘤发生。衰老细胞会在肿瘤微环境中分泌多种促炎因子，这些因子能够支持肿瘤细胞的存活、增殖与迁移。本研究对Ptenpc-/-衰老肿瘤组织及Ptenpc-/-;Trp53pc-/-非衰老肿瘤组织中的细胞因子及细胞因子相关因子的基因表达情况进行了检测。RNA测序（RNAseq）分析证实，在Ptenpc-/-衰老肿瘤组织中存在一类特异性上调和下调的细胞因子，即“核心衰老相关分泌表型（core-SASP）”。本研究同时还发现了一批上调的分泌因子，这些因子与细胞衰老无关，且在Ptenpc-/-与Ptenpc-/-;Trp53pc-/-两种肿瘤组织中均有表达。