Additional file 1 of Ubiquitin-specific protease 7 is a drug-able target that promotes hepatocellular carcinoma and chemoresistance

Additional file 1. a HuH7 and SK-Hep1 stable cells were used for 2 weeks. The number of colonies were counted and stained with crystal violet. b Anchor-dependent colony formation was measured after 2 weeks. c Cell viability was measured with CCK-8 assays in SK-Hep1 and Huh7 cells. d The expression of USP7 was detected by Western Blot in HuH7 and SK-Hep1 stable cells. e SK-Hep1 stable cells were prepared and stained with PI and Annexin V. Cells were analyzed using a flow cytometry. The apoptotic percentage: left (UR 1.99, LR 1.78), middle (UR 3.88, LR 4.16), right (UR 3.27, LR 6.69). f The effect of USP7 deletion on cell migration was examined by wound healing assay.

附加文件1。a. 以HuH7与SK-Hep1稳定细胞株培养2周后，对细胞集落进行计数并以结晶紫染色。b. 锚定依赖性集落形成能力于培养2周后开展检测。c. 采用CCK-8法检测SK-Hep1与Huh7细胞的细胞活力。d. 通过蛋白质印迹法（Western Blot）检测HuH7与SK-Hep1稳定细胞株中USP7的表达水平。e. 制备SK-Hep1稳定细胞株，以碘化丙啶（PI）与膜联蛋白V（Annexin V）染色后，借助流式细胞术（flow cytometry）分析细胞。细胞凋亡比例：左侧组（右上象限UR 1.99、左下象限LR 1.78）、中间组（UR 3.88、LR 4.16）、右侧组（UR 3.27、LR 6.69）。f. 通过划痕愈合实验（wound healing assay）检测USP7敲除对细胞迁移能力的影响。