Transcriptomic minimal dataset.

The dysregulation of phosphatidylcholine (PC), triglycerides (TG), phosphatidylethanolamine (PE), and cardiolipin (CL) metabolism is believed to contribute to the development of MASLD. However, little is known about the mechanisms underlying the onset of this condition. To establish a mouse model of MASLD, C57BL/6J mice were fed a high-fat diet (HFD). Lipidomics was applied to identify differences in liver lipids. RNA-sequencing and bioinformatics analyses were conducted to investigate changes in the expression of genes and pathways associated with these metabolic processes. 49 lipid classes and 3221 lipid species were identified using positive- and negative-ion pattern identification. A total of 678 differentially expressed genes were identified, of which 364 were upregulated and 314 were downregulated in the MASLD group. KEGG enrichment pathway analysis highlighted the downregulation of four genes such as Gpat4, Gpcpd1, Chkb, and Etnppl. These findings contribute to our understanding of the metabolic changes associated with MASLD.

现有研究表明，磷脂酰胆碱（phosphatidylcholine, PC）、甘油三酯（triglycerides, TG）、磷脂酰乙醇胺（phosphatidylethanolamine, PE）及心磷脂（cardiolipin, CL）的代谢失调与代谢功能障碍相关性脂肪性肝病（Metabolic Dysfunction-Associated Steatotic Liver Disease, MASLD）的发生发展密切相关，但目前对该疾病的发病机制仍知之甚少。为构建MASLD小鼠模型，本研究通过高脂饮食（high-fat diet, HFD）喂养C57BL/6J小鼠。采用脂质组学（lipidomics）分析鉴定肝脏脂质的表达差异，并通过RNA测序（RNA-sequencing）及生物信息学分析，探究与上述代谢过程相关的基因及通路的表达变化。本研究通过正、负离子模式鉴定，共识别出49个脂质类别及3221种脂质分子。最终共鉴定得到678个差异表达基因，其中MASLD模型组中有364个基因上调、314个基因下调。KEGG富集通路分析显示，Gpat4、Gpcpd1、Chkb及Etnppl等4个基因呈现显著下调趋势。本研究结果为深入理解MASLD相关代谢变化提供了重要参考。