Microarray Analysis of MicroRNA Expression in Peripheral Blood Mononuclear Cells of critically ill Patients with Influenza A (H1N1). Homo sapiens

To determine whether differential expression of cellular microRNAs plays a role in the host response to Influenza A (H1N1) infection, we have employed the Agilent miRNA microarray (V3) as a discovery platform to identify microRNAs between the critically ill Patients with Influenza A (H1N1) and the healthy controls. Overall design: Five critically ill patients with a diagnosis of 2009 Inflluenza A (H1N1) and three healthy controls were included in the study. The Peripheral Blood Mononuclear Cells (PBMCs) were isolated and total RNA was extracted respectively.

为探究细胞微小核糖核酸（microRNAs）的差异表达是否参与宿主对甲型流感（H1N1）感染的应答过程，本研究采用Agilent miRNA微阵列（V3）作为发现平台，对重症甲型流感（H1N1）患者与健康对照者之间的差异表达微小核糖核酸进行筛选。实验整体设计：本研究纳入5例经确诊的2009年甲型流感（H1N1）重症患者及3例健康对照者，分别分离其外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMCs）并提取总RNA。