Genome-wide map of p53 binding sites by ChIP-seq in human lymphoblastoid cell lines treated with nutlin-3. Homo sapiens

We have used chromatin immune-precipitation with parallel sequencing (ChIP-Seq) technology to identify genome-wide p53 binding in human lymphoblastoid cell lines treated with a MDM2 inhibitor nutlin-3 Overall design: ChIP-Seq analysis of p53 binding sites in human lymphoblastoid cells treated with nutlin-3 or vehicle

本研究采用染色质免疫共沉淀测序（chromatin immune-precipitation with parallel sequencing, ChIP-Seq）技术，对经MDM2抑制剂nutlin-3处理的人类淋巴母细胞系中的全基因组p53结合位点进行鉴定。实验设计：对经nutlin-3或溶剂对照处理的人类淋巴母细胞进行p53结合位点的ChIP-Seq分析。