RNA-seq of organ of Corti RNA from mice carrying a null allele of Mir183 and Mir96. RNA-seq of organ of Corti RNA from mice carrying a null allele of Mir183 and Mir96

The microRNA miR-96 is important for hearing; mutations in the seed region result in dominant progressive hearing loss in mice and humans. Mir96 is expressed in the sensory hair cells of the organ of Corti along with Mir182 and Mir183. miR-96 is a master regulator of hair cell development, controlling many genes in the organ of Corti, but the role of miR-182 and miR-183 in the hair cells is unknown. We carried out RNA-seq on mice carrying a knockout allele of Mir182, and mice carrying a double knockout allele of Mir183 and Mir96 (Mir183/96). RNA was extracted from the organ of Corti from P4 homozygotes and sex-matched wildtype littermates. Strand-specific libraries were prepared using the NuGEN Ovation Mouse RNA-Seq System 1-16 kit and sequenced on an Illumina HiSeq 2500 machine as paired-end 125bp reads.

微小RNA（microRNA）miR-96在听觉功能中具有关键作用；其种子区的突变可引发小鼠与人类出现显性进行性听力损失。Mir96与Mir182、Mir183共同在Corti器的感觉毛细胞中表达。miR-96是毛细胞发育的主调控因子，能够调控Corti器内的众多基因，但目前尚不清楚miR-182与miR-183在毛细胞中所发挥的生物学功能。本研究对携带Mir182单敲除等位基因的小鼠，以及同时敲除Mir183与Mir96的双敲除等位基因小鼠（Mir183/96）开展了RNA测序（RNA-seq）。实验材料取自出生后第4天（P4）的纯合突变小鼠与性别匹配的野生型同窝幼鼠的Corti组织，从中提取总RNA。采用NuGEN Ovation Mouse RNA-Seq System 1-16试剂盒构建链特异性文库，并在Illumina HiSeq 2500测序平台上以125bp双端读长进行测序。