Investigating cellular signaling proteins and complexes using time-resolved serial crystallography.

For many years members of the Laboratory of Biomolecular Research have been driving the development of synchrotron based serial crystallography including
pioneering static (Nogly et al., 2015 at ESRF, Weinert et al., 2017) and time-resolved experiments (Nogly et al., 2016, Weinert et al., 2019, Skopintsev et al.,
2022, Weinert et al., 2023) at SLS and SwissFEL. With the coming upgrade of the SLS and an expected shutdown of 2 years, we are submitting a BAG continuation proposal
in order to continue our research projects at the ESRF. Our interests span photoactivation and ligand un-/binding in G protein-coupled receptors, kinase activation,
temperature dependence of enzyme activity and multi-domain photosensors. X-ray beam brilliance, experienced beamline staff and the variety of available sample
delivery methods make the ID29 beamline an ideal place to conduct the proposed projects which have already strengthened collaboration between two
centers of European X-ray science.

多年来，生物分子研究实验室（Laboratory of Biomolecular Research）的科研人员一直推动基于同步辐射的串行晶体学（synchrotron-based serial crystallography）的发展，相关工作涵盖开创性静态实验与时间分辨实验：其中Nogly等2015年的静态实验于欧洲同步辐射装置（ESRF）开展，Weinert等2017年的静态实验及Nogly等2016、Weinert等2019、Skopintsev等2022、Weinert等2023年的时间分辨实验均在瑞士光源（SLS）与瑞士自由电子激光（SwissFEL）上完成。鉴于瑞士光源即将进行升级并预计关停两年，我们正提交光束线分配组（BAG）延续申请，以期能在欧洲同步辐射装置（ESRF）继续推进我们的研究项目。我们的研究方向涵盖G蛋白偶联受体（G protein-coupled receptors, GPCR）的光激活与配体结合/解离过程、激酶激活、酶活性的温度依赖性以及多域光传感器的相关研究。X射线束流亮度、经验丰富的光束线运维人员以及多样的样品递送方案，使得ID29光束线成为开展本次申请项目的理想场所；该项目已推动欧洲两家X射线科学研究中心的合作深化。