Mating loci and meiosis genes and CDS of Urocystis primulicola

Core meiosis genes/CDS were manually annotated in the genome of <em>Urocystis primulicola</em> using the respective genes from <em>Ustilago maydis</em> (Gioti et al., 2013). Mating loci and mating genes/CDS were identified using the mating genes of <em>Ustilago maydis</em> and <em>Sporisorium reilianum</em>. The dataset contains the sequences in GenBank format for the meiosis genes and both mating loci conserved in Ustilaginomycetes. This dataset was generated in the study described in the unpublished manuscript "Comparative genomics of smut fungi suggest the ability of meiosis and mating in asexual species of the genus <em>Pseudozyma</em> (Ustilaginales)". Accession numbers of reference genes are cited in the manuscript. Tblastn was used to identify the loci in the genome, and multiple sequence alignments (MUSCLE) of the respective reference gene and CDS with the suspected locus were used to identify the reading frames and intron positions.

本研究以玉米黑粉菌（Ustilago maydis）的同源基因为参照（Gioti等人，2013年），在报春黑粉菌（Urocystis primulicola）的基因组中对核心减数分裂基因及编码序列（Coding Sequence, CDS）进行了手动注释。交配位点及交配相关基因/编码序列则以玉米黑粉菌和高粱丝黑穗病菌（Sporisorium reilianum）的交配基因为参照完成鉴定。本数据集包含黑粉菌纲（Ustilaginomycetes）中保守的减数分裂基因与两类交配位点的GenBank格式序列。本数据集源自未发表手稿《黑粉菌比较基因组学揭示假丝酵母属（Pseudozyma，黑粉菌目）无性物种具备减数分裂与交配能力》中的相关研究。参考基因的登录号已在该手稿中注明。研究采用Tblastn工具在基因组中定位目标位点，并通过将候选位点与对应参考基因及编码序列进行多重序列比对（MUSCLE），以确定开放阅读框与内含子位置。