Dataset for: Distribution of Bactericera cockerelli in Ecuador

A field study was carried out in two important potato producing provinces of Ecuador, Imbabura and Cotopaxi, to: (1) identify distribution and abundance of potato psyllid (PP) Bactericera cockerelli over time, (2) perform molecular characterization and determine potato psyllid haplotype(s), and (3) use the Insect Life Cycle Modeling (ILCYM) software for pest-risk mapping and assessment. The metadata in these databases correspond to the first study objective in the monitoring of Bactericera cockerelli. Farm plots measuring 1000m2 by 1000m2 were selected from each canton/parish per province and evaluated for presence of psyllid eggs, nymphs and adults during two potato farming cycles; the number of evaluations performed were adjusted according to sample size. 20 plants were randomly selected transeptally in the form of an X from each field site per evaluation of psyllid eggs and nymphs respectively, in 15-day intervals. Using a magnifying glass 5X, plants collected for psyllid egg analysis were divided in quadrants and scanned in order, starting at the apex, while six leaves from each of the plants collected for psyllid nymph analysis were used to quantify the number of IV and V stage nymphs. Data collected was registered in the field logbook (data in the “eggs_month_evaluation” sheet and "nymphs_month_evaluation"sheet, respectively). Monitoring of adults was performed using two HORIVER traps allocated in each field, one on the edge and one in the middle. Traps were collected and replaced every 15 days and sent to the DNPV laboratory for identification and quantification; date of evaluation, farm plot location and coordinates were also registered (data in the “adults_month_evaluation” sheet). Data logger devices were also placed in the nearest canton/parish to the selected field plots in order to register soil temperature, and were monitored over a similar interval of days (data in the “Wheather_field_experiment” sheet). A onetime collection of adult specimens was also carried out at each monitoring site by entomological nets (data in the "entomofauna_month_evaluation" sheet. Secondary information such as the phenological state of potato crops, agronomic cultural practices, fertilization, irrigation and precipitation, and phytosanitary controls performed prior to each evaluation were also compiled (data in the "other_variables_evaluations" sheet and "phytosanitary_control_evaluations" sheet. Finally, an aggregate database which includes eggs, nymphs and adults evaluations of Bactericera cockerelli was crossed with temperatura (data in "All_Database_t") and a second aggregated database with the average numbers of eggs, nymphs and adults (data in "All_database_treduced")

本研究在厄瓜多尔两个重要马铃薯产区省份——因巴布拉省（Imbabura）与科托帕希省（Cotopaxi）开展田间试验，旨在达成三大研究目标：（1）明确马铃薯木虱（potato psyllid, PP）*Bactericera cockerelli*的时空分布与种群丰度；（2）开展分子鉴定并确定该马铃薯木虱的单倍型；（3）利用昆虫生命周期建模（Insect Life Cycle Modeling, ILCYM）软件开展害虫风险制图与评估。本数据集的元数据对应上述第一项研究目标，即针对*Bactericera cockerelli*的监测调查。研究人员在每个省份的各区县/教区选取面积为1000m×1000m的农田样地，在两个马铃薯种植周期内开展木虱卵、若虫与成虫的调查，并根据样本量调整调查频次。针对卵与若虫的调查，每次均采用X形随机抽样法在每个样地内选取20株植株，调查间隔为15天。使用5倍放大镜开展卵调查时，将采集植株按象限划分并从植株顶端开始依次扫描；开展若虫调查时，则从每株采集植株上摘取6片叶片，用于统计4龄与5龄若虫的数量。相关调查数据分别记录于田间日志的"eggs_month_evaluation"与"nymphs_month_evaluation"工作表中。成虫监测采用2个HORIVER诱捕器，分别放置于样地边缘与样地中部。诱捕器每15天更换并回收，随后送至DNPV实验室进行种类鉴定与种群数量统计；同时记录调查日期、农田样地位置与坐标，相关数据记录于"adults_month_evaluation"工作表中。研究人员在所选样地附近的区县/教区部署数据记录仪以记录土壤温度，监测间隔与调查周期一致，相关数据记录于"Wheather_field_experiment"工作表中。此外，还使用昆虫网在每个监测点一次性采集成虫标本，相关数据记录于"entomofauna_month_evaluation"工作表中。本研究还收集了每次调查前马铃薯作物的物候期、农艺栽培措施、施肥、灌溉、降水情况以及植物检疫措施等辅助信息，相关数据分别记录于"other_variables_evaluations"与"phytosanitary_control_evaluations"工作表中。最终，将包含*Bactericera cockerelli*卵、若虫、成虫调查数据的整合数据库与温度数据（记录于"All_Database_t"工作表）进行关联整合；同时构建第二套整合数据库，收录卵、若虫、成虫的平均数量数据，相关内容记录于"All_database_treduced"工作表中。