Porcine neutrophils infected by Salmonella

High-density gene expression profile of 14,151+ unique genes for 6 normal porcine neutrophild and 6 porcine neutrophils samples infected by Salmonella using deep sequencing technology. Overall design: Peripheral blood samples were collected from 20 healthy crossbreed (Duroc × Landrace × Yorkshire) piglets from a commercial swine farm in Jingzhou, China without sacrificing the animals (ten males and ten females, four weeks old). Prior to blood extraction, the feces were tested negative for Salmonella three times. Neutrophils were isolated through density gradient centrifugation using Ficoll-PaqueTM media (GE Healthcare Life Sciences, Shanghai) as per manufacturer instructions. Luria broth and M9 minimal medium were used to culture Salmonella bacteria. The neutrophils were treated with 106 colony forming units (cfu) of Salmonella enterica serovar Typhimurium (ATCC® 14028™) as previously described [7]. Samples were collected at zero- or eight-hours post infection (hpi). Six samples from each group (zero and eight hpi) were selected for transcriptome analysis.

本数据集采用深度测序技术，对6份正常猪中性粒细胞（porcine neutrophil）样本及6份沙门氏菌（Salmonella）感染猪中性粒细胞样本的14151+个独特基因开展高密度基因表达谱分析。实验设计概况：从中国荆州某商业养猪场采集20头健康杂交（杜洛克×长白×约克夏）仔猪的外周血样本，采血过程未对动物实施宰杀，受试仔猪为公母各10头、4周龄。采血前，通过三次粪便检测确认仔猪沙门氏菌呈阴性。采用Ficoll-Paque™ 培养基（通用电气医疗生命科学，上海），按照制造商说明书通过密度梯度离心法分离中性粒细胞。使用LB肉汤（Luria broth）和M9基本培养基培养沙门氏菌。参照已发表文献[7]的方法，用10^6菌落形成单位（colony forming units, cfu）的鼠伤寒沙门氏菌（Salmonella enterica serovar Typhimurium，ATCC® 14028™）处理分离得到的中性粒细胞。分别在感染后0小时和8小时（hpi）收集样本。每组（0 hpi和8 hpi）各选取6份样本进行转录组分析（transcriptome analysis）。