Summary of the localization, vesicle-mediated transport steps, vacuole fusion, and Syb1 localization in membrane trafficking defective mutants.

*Group I indicates that similar to wild-type cells GFP-Syb1 was visibly seen on the plasma membrane with more prominence at the growing ends of the cells.
Group II indicates that GFP-Syb1 accumulated as large dot-like structures in the cytoplasm.
Group III indicates that GFP-Syb1 localized almost evenly all around the cell surface, and the punctate structures in the cytoplasm were barely observed.
Group IV indicates that GFP-Syb1 failed to localize to the membrane and the fluorescence was weak and hazy.

* 组别I：与野生型细胞一致，GFP-Syb1可在质膜上被观测到，且在细胞生长末端的荧光信号更为显著。
* 组别II：GFP-Syb1在细胞质中聚集为大型点状结构。
* 组别III：GFP-Syb1几乎均匀分布于整个细胞表面，细胞质内几乎未见点状结构。
* 组别IV：GFP-Syb1无法定位于膜结构，荧光信号微弱且弥散。