Total RNA-seq of siRNA-mediated SRSF1 knockdown

To identify changes in splicing patterns following SRSF1 knockdown, RNA-sequencing was performed on breast cancer cells transfected with siRNAs targeting SRSF1. Overall design: Libraries were generated using ScriptSeq v2 RNA-seq Library Preparation Kit (Epicentre) and run on Illumina HiSeq 2500.

为鉴定SRSF1基因敲低后剪接模式的变化，本研究对转染靶向SRSF1的小干扰RNA（siRNAs）的乳腺癌细胞开展RNA测序（RNA-sequencing）。实验总体设计：使用Epicentre公司的ScriptSeq v2 RNA测序文库制备试剂盒构建测序文库，并在Illumina HiSeq 2500测序平台上完成测序。