Comparison of gene expression profilings between parental A549, OSK-A549-Colony and OSK-A549-SN

In the present study, we successfully generated lung cancer stem cell (CSC)-like cells by introducing a small set of transcription factors into a lung cancer cell line (OSK-A549-Colony). In addition to properties that are conventionally referred to as CSC properties, the lung induced CSCs exhibited the ability to form lung cancer-like tissues in vitro with vascular cells and mesenchymal stem cells, which showed structures and immunohistological patterns that were similar to human lung cancer tissues. We extracted total RNA from 3 lung cancer cell lines (parental A549), 3 induced lung cancer stem like cells (OSK-A549-Colony) and 3 differentiated non lung cancer stem like cells (OSK-A549-SN) and hybridized them to Agilent gene expression microarrays.

本研究通过向肺癌细胞系OSK-A549-Colony中导入少量转录因子，成功诱导获得了肺癌干细胞（cancer stem cell, CSC）样细胞。除具备常规定义的肺癌干细胞特性外，该诱导所得的肺癌干细胞样细胞还可与血管细胞及间充质干细胞共同在体外构建类肺癌组织，其组织结构与免疫组化特征均与人类肺癌组织高度相似。我们分别从3株亲本肺癌细胞系（亲本A549）、3株诱导型肺癌干细胞样细胞（OSK-A549-Colony）以及3株分化后的非肺癌干细胞样细胞（OSK-A549-SN）中提取总RNA，并将其与安捷伦（Agilent）基因表达芯片进行杂交实验。