RNA sequencing and integrative analysis reveal pathways and hub genes associated with TGFβ1 stimulation on prostatic stromal cells (PrSCs)

To examine the effect of TGFβ1 on prostatic stromal cells, we firstly isolated primary prostatic stromal cells from five benign prostatic hyperplasia sample. Then RNA sequencing and bioinformatics analysis were used to reveal important pathways and hub genes associated with TGFβ1 stimulation on PrSCs. Comparative analysis of RNA-seq data for primary prostatic stromal cells with and without TGFβ1 stimulation

为探究转化生长因子β1（transforming growth factor β1, TGFβ1）对前列腺基质细胞的调控效应，我们首先从5例良性前列腺增生样本中分离得到原代前列腺基质细胞。随后借助RNA测序（RNA sequencing, RNA-seq）与生物信息学分析方法，揭示了TGFβ1刺激前列腺基质细胞（prostatic stromal cells, PrSCs）相关的关键通路与核心基因。本数据集针对经与未经TGFβ1刺激的原代前列腺基质细胞的RNA-seq数据开展对比分析。