ZAP affects Zika virus RNA interactome- supplemental datasets 1 and 2

One of the most recent advances in the analysis of viral RNA–cellular protein interactions is the Comprehensive Identification of RNA-binding Proteins by Mass Spectrometry (ChIRP-MS). Here, we used ChIRP-MS in mock-infected and Zika-infected wild-type cells and cells knockout for the zinc finger CCCH-type antiviral protein 1 (ZAP). We characterized “ZAP-independent” and “ZAP-dependent” cellular protein interactomes associated with flavivirus RNA and found that ZAP affects cellular proteins associated with Zika virus RNA. The ZAP-dependent interactome identified with ChIRP-MS provides potential ZAP co-factors for antiviral activity against Zika virus and possibly other viruses. Identifying the full spectrum of ZAP co-factors and mechanisms of how they act will be critical to understanding the ZAP antiviral system and may contribute to the development of antivirals., , , # ZAP affects Zika virus RNA interactome - supplemental datasets 1 and 2

[https://doi.org/10.5061/dryad.kwh70rzd1](https://doi.org/10.5061/dryad.kwh70rzd1)

Supplementary data

## Description of the data and file structure

**Table S1** **- ChIRP-MS data**

Table S1 ChIRP_MS data, **sheet** “**C Extended Zika interactome**” – Proteins with top 15 Spectral Counts are highlighted in red. RNA helicases are highlighted in yellow.

Table S1 ChIRP_MS data, **sheet** “**D Reference Ooi et al., 2019**” – The same proteins in both studies are highlighted in red.

Table S1 ChIRP_MS data, **sheet** “**F ZAP-independent interactome**” – Proteins with top 15 Spectral Counts are highlighted in red.

Table S1 ChIRP_MS data, **sheet** “**H ZAP-dependent interactome**” – Proteins with top 15 Spectral Counts are highlighted in red. RNA helicases are highlighted in yellow.

The empty cells in files are a result of the programs used.

**Supplemental Materials and Methods.** The file contains experimental ...

在病毒RNA与细胞蛋白相互作用分析领域，最新进展之一是质谱法全面鉴定RNA结合蛋白技术（ChIRP-MS）。本研究在模拟感染和寨卡病毒感染的野生型细胞及锌指CCCH型抗病毒蛋白1（ZAP）敲除细胞中应用ChIRP-MS技术，表征了与黄病毒RNA相关的“不依赖ZAP”和“依赖ZAP”的细胞蛋白相互作用组（interactome），发现ZAP可影响与寨卡病毒RNA结合的细胞蛋白。通过ChIRP-MS鉴定的依赖ZAP的相互作用组为抗寨卡病毒及其他潜在病毒的ZAP辅助因子提供了候选靶标。明确ZAP辅助因子的完整谱图及其作用机制，对理解ZAP抗病毒系统至关重要，并可能为抗病毒药物研发提供助力。,,, # ZAP影响寨卡病毒RNA相互作用组 - 补充数据集1和2

[https://doi.org/10.5061/dryad.kwh70rzd1]

补充数据

数据与文件结构描述

**表S1** **- ChIRP-MS数据**

表S1 ChIRP-MS数据，**工作表**“**C 扩展寨卡病毒相互作用组**”——光谱计数（Spectral Counts）前15的蛋白以红色标注，RNA解旋酶以黄色标注。

表S1 ChIRP-MS数据，**工作表**“**D 参考文献Ooi等，2019**”——两项研究中共同存在的蛋白以红色标注。

表S1 ChIRP-MS数据，**工作表**“**F 不依赖ZAP的相互作用组**”——光谱计数前15的蛋白以红色标注。

表S1 ChIRP-MS数据，**工作表**“**H 依赖ZAP的相互作用组**”——光谱计数前15的蛋白以红色标注，RNA解旋酶以黄色标注。

文件中的空单元格为所用分析程序的输出结果。

**补充材料与方法**：该文件包含实验...