Fusion junctions are confirmed by PGM sequencing of PCR amplicons.
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#In these 7 PGM libraries containing a single PCR reaction with an unique PGM barcode, the fusion amplicons identify the most prevalent clonal population in the library. The detailed experimental results including amplicon sequences are in Table S3.
在这7个个人基因组测序仪(PGM)文库中,各文库均包含单次聚合酶链式反应(PCR)及唯一PGM条形码,融合扩增子可识别其中占比最高的克隆种群。包含扩增子序列在内的详细实验结果详见表S3。
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2015-12-02



